| Gene name: | CIRCPVT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Western blot |
| Description: | Silencing CircPVT1 led to increased levels of senescence marker TP53(p53) and triggered a flattened and enlarged cell morphology accompanied by increased SA-β gal activity. |
| Target gene: | LET-7 |
| Official symbol(s): | LET-7 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//SA-β-gal activity assay//Cell morphological analysis//Western blot//Dual-Luciferase reporter assay |
| Target gene description: | One of the microRNAs,let-7, was found to be the most highly enriched microRNA in the CircPVT1 pulldown.Importantly, silencing CircPVT1, which increases let-7 availability, significantly reversed this loss of P21 and TP53 expression observed with anti-let-7 alone.SA-βgal activity was elevated in CircPVT1-silenced cells, while anti-let-7 had the opposite effect, reducing SA-βgal activity and increasing proliferation; importantly, the proliferative phenotype seen after antagonizing let-7 was rescued when CircPVT1 was silenced. Although pre-let-7-transfected cells showed greater reduction in psiCHECK2-let-7 activity(RL/FL) (45% of control), silencing CircPVT1 also significantly lowered RL/FL activity (70% of control),indicating that CircPVT1 reduced the availability of functional let-7 to transcripts bearing a let-7 site. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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