| Gene name: | MIR1292 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Adipose stromal cell |
| Gene ID: | 100302138 |
| Category: | ncRNA |
| Phenotype: | Age-related osteoporosis |
| Experimental category: | L |
| PMID: | 30574422 |
| Experiment: | qRT-PCR//Western blot//SA-β-gal activity assay |
| Description: | qRT-PCR and western blot analyses also showed that lenti-1292-infected hADSCs expressed higher levels of the senescence markers P16 and P21 and lower expression of LMNB1 than control cells.Moreover, the number of SA-β-gal-stained cells was increased after miR-1292 overexpression. |
| Target gene: | FZD4 |
| Official symbol(s): | FZD4 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//qRT-PCR//Western blot |
| Target gene description: | Results showed that miR-1292 overexpression remarkably inhibited luciferase reporter activity from the vector containing the WT FZD4 3′ UTR, but not from the mutated FZD4 or those of other genes. Additionally, after miR-1292 overexpression, FZD4 was markedly down -regulated at the protein level, but not at mRNA level, Also, qRT-PCR results showed that the expression of miR-1292 was negatively correlated with FZD4 in 70 clinical bone samples. |
| Regulatory pathway: | WNT-β-CATENIN |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | Wnt-CTNNB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Western blotting studies revealed that miR-1292 overexpression decreased both FZD4 and β-catenin expression, indicating that Wnt/β-catenin signaling was impaired. |
Annotation:
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