| Gene name: | MIR15B |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF |
| Experiment: | qRT-PCR |
| Description: | We observed an increased expression at day 2 upon transfection of miR-15b inhibitors followed by a clear decrease of transcript levels (31-80%) at day 3 (IFNγ) and day 4 (IL-1α, IL-1β, IL-6, IL-8, and VEGF). |
| Target gene: | SIRT4 |
| Official symbol(s): | SIRT4 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT‐PCR//Luciferase reporter assay |
| Target gene description: | Similarly, and again in accordance with the SIRT4 expression status,miR-15b levels were also decreased in UV- irradiated or gamma-irradiated senescent primary human dermal fibroblasts.In these experiments, miR-15b mimics dampened basal SIRT4 mRNA levels and inhibited senescence-associated upregulation of SIRT4 transcripts.Moreover, by employing luciferase assays we show that the 3’UTR of human SIRT4 is a direct target of miR-15b, as miR-15b mimics significantly inhibited the relative luciferase activity of a transfected SIRT4 - 3’-UTR construct, but not, when the SIRT4 – 3’-UTR was mutated within the seed sequence for miR-15b. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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