| Gene name: | MIR494 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay//Cell cycle analysis//Western blot//Cell morphological analysis |
| Description: | Gain of miR-494 in early-passage HUVECs increased senescence-associated β-galactosidase levels (SA-β-gal).Ectopic expression of miR-494 increased G1 arrest, whereas the inhibition of the miR decreased the G2 arrest in response to a 10?Gy dose of radiation .miR-494 impacted other hallmarks of senescence—a decrease in telomerase activity , an increase in the cell cycle regulator p21 as well as a decrease in Rb hyperphosphorylation .phalloidin staining in HUVECs after 48?h of miR-494 treatment also revealed flattened and multinucleated cells. |
| Target gene: | MRN |
| Official symbol(s): | MRN |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot//Immunostaining//Luciferase reporter assay |
| Target gene description: | Transfection of miR-494 decreased both MRN RNA and protein levels,miR-494 decreased activity of a luciferase reporter cloned upstream of each 3’-UTR of the MRN complex genes . miR-494-transfected HUVECs also showed a decrease in MRE11a nuclear foci. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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