| Gene name: | MIR141 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Umbilical cord blood |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | In the miR-141-3p-transfected cells, MTT assays and cell cycle analyses revealed that the growth rate was reduced 48?hours post-transfection. In contrast, the transfection of hMSCs with anti-miR-141-3p yielded an increase in proliferation. The correlation between the expression of ZMPSTE24 and miR-141-3p was confirmed in five other hMSC cell lines .The overexpression of miR-141-3p also increased SA-β-gal activity and γH2Ax expression in these cells. |
| Target gene: | ZMPSTE24 |
| Official symbol(s): | ZMPSTE24 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | We further investigated whether miR-141-3p regulates ZMPSTE24 expression, prelamin A accumulation and nuclear abnormalities. The transfection of hMSCs with miR-141-3p decreased ZMPSTE24 expression, resulting in increased expression of prelamin A, p16INK4A?and γH2AX. Conversely, these effects were reversed by miR-141-3p knockdown. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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