| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | UM-SCC-23,Fadu |
| Gene ID: | 407040 |
| Category: | ncRNA |
| Phenotype: | Squamous cell carcinoma |
| Experimental category: | HL |
| PMID: | 28485160 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Compared with the wide type and control cells, the miR-34a over-expression cells exhibited an about 1.6 to 2.0 times increase in the percentage of cell at G0/G1 phase, along with a?decreased cell percentage at the S?phase.The result showed that the mean percentage of SA-β-gal-positive cells increased by 4.41 times (UM-SCC-23-miR-34a) and 8.18 times (Fadu-miR-34a) in miR-34a over-expression cell lines than that in control cell lines. |
| Target gene: | MAP2K1//AXL//FUT1//AREG |
| Official symbol(s): | MAP2K1//AXL//FUT1//AREG |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//qRT-PCR |
| Target gene description: | The result indicated that the mRNA levels of 8 genes were reduced about 1.4-fold to 4.7-fold in cells with ectopic miR-34a expression, compared with control.The luciferase reporter activity of pSiCheck?-2-AXL-3’UTR, pSiCheck?-2-FUT1-3’UTR and pSiCheck?-2-MAP2K1-3’UTR was markedly reduced by forced expression of miR-34a compared with the control(2.43- fold decrease, 2.08-fold decrease and 4.78-fold decrease,respectively), whereas no reporter activity were found when their target sites were mutated. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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