| Gene name: | MIR21 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U87 |
| Experiment: | SA-β-gal activity assay//Knockdown//MTT assay |
| Description: | Compared with the blank and NC groups, the miR-21 mimics and siRNA-SPRY1 groups revealed a decreased density of U87 cells and enlarged cell size, in addition to reduced senescence indexes. In the miR-21 inhibitors group, the senescence index of U87 cells was increased; and no significant difference in senescence index was identified in the miR-21 inhibitors + siRNA-SPRY1 group.MTT results indicated that, compared with the blank and NC groups, cell proliferation reduced in the miR-21 inhibitors group and increased in the miR-21 mimics and siRNA-SPRY1 group; there were no significant differences in cell proliferation in the miR-21 inhibitors + siRNA-SPRY1 group. |
| Target gene: | SPRY1 |
| Official symbol(s): | SPRY1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Dual-Luciferase reporter assay//qRT-PCR |
| Target gene description: | The results of the dual-luciferase reporter assay system indicated that there was a significant reduction in the luciferase signal in SPRY1-wt cotransfection group, compared with other miR-21 transfection groups. And, there was no difference in the luciferase signal of SPRY1-mut among miR-21 transfection groups.The results of RT-qPCR showed that, compared with the control group, mRNA levels of miR-21, PI3K, and AKT increased in the glioma group, but mRNA levels of SPRY1 and PTEN decreased. |
| Regulatory pathway: | PTEN-PI3K-AKT |
| R-AG-Pathway: | -- |
| Official symbol(s): | PTEN-PIK3CA-AKT1 |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | In the miR-21 mimics and siRNA-SPRY1 groups, protein levels of PI3K, AKT, p-AKT, P53, and p-GSK3 increased, and those of SPRYI1, PTEN, Caspase-3, and Caspase-9 decreased;and the miR-21 inhibitors group exhibited opposite trends; in the miR-21 mimics group, miR-21 expression and mRNA expressions of PI3K and AKT increased while mRNA levels of SPRY1 and PTEN decreased . |
Annotation:
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