| Gene name: | MIR22 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | EPC |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | Using β-galactosidase expression as an indicator of cell senescence, we found that overexpression of miR-22 in Y-EPCs increased senescence.Overexpression of miR-22 in Y-EPCs inhibited proliferation. |
| Target gene: | AKT3 |
| Official symbol(s): | AKT3 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot//RT-PCR |
| Target gene description: | miR-22 overexpression decreased the relative luciferase activity of the 3′UTR reporter, whereas it had no effect on the mutant 3′UTR reporter, indicating that the intact seed sequence is required for miR-22 functionality.miR-22 overexpression in Y-EPCs significantly downregulated AKT3 mRNA and protein expression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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