| Gene name: | RP11-670E13.6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF |
| Experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry//Cell cycle analysis |
| Description: | RP11-670E13.6 expression was knocked down using an siRNA, which significantly decreased the proliferation of HDFs.Our analysis revealed that RP11-670E13.6-depleted HDFs showed elevated SAβ-gal activity, a distinguishing marker of senescent cells, but no significant effects on apoptosis were observed.RP11-670E13.6 knockdown significantly inhibited cell cycle progression, as evidenced by a significant increase in the G0/G1 fraction and a significant decrease in G2/M cells. |
| Regulatory pathway: | P16-PRB |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN2A-RB1 |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | RP11-670E13.6 knockdown increased expression of p16，RP11-670E13.6 knockdown promoted cell cycle arrest and senescence, perhaps through the p16-pRB pathway, independently of the p53-p21 pathway。Knockdown of RP11-670E13.6 had a significant effect on cell senescence-associated mRNA expression. Knockdown of RP11-670E13.6 had a significant effect on cell senescence associated protein expression. |
Annotation:
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