| Gene name: | NANOG |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | NIH-3T3 MEF,Mouse Fibroblast cell |
| Experiment: | SA-β-gal activity assay//Cell proliferation assay |
| Description: | Nanog-TAT caused a strongly increased proliferation, yielding about four-fold more cells within 10 days as compared to the control.Approximately 6% of MP-hADFs cultured under normal conditions for 3 passages stained positive for SA-β-gal. In contrast, no SA-β-gal activity was detectable in MPhADFs cultured in the presence of Nanog-TAT. |
| Target gene: | P27 |
| Official symbol(s): | P27 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | After 5 h of Nanog-TAT treatment, the RNA expression level of p27KIP1 was slightly reduced, to ～90% compared to fibroblasts cultured in control medium. After 8 h of Nanog-TAT application, the expression of p27KIP1 was diminished to～70%. After 21 h of Nanog transduction, we detected only ～25% p27KIP1 transcript as compared to cells incubated with control medium.Quantification demonstrates that 5 h of Nanog transduction is sufficient to reduce the p27KIP1 protein level to about half compared to control; 24 h of incubation with Nanog-TAT yielded a further decline of p27KIP1 to ～40% compared to control. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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