| Gene name: | PEBP1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | hTERT-immortalized p21?/?,LF1,PC-3,HCT116 |
| Experiment: | Knockdown//SA-β-gal activity assay//MTT assay//Western blot |
| Description: | Elimination of RKIP could increase ERK activation and suppress the cellular senescence. Conversely, RKIP overexpression inhibited ERK activation and induced the cellular senescence (monitored by SA-β-Gal and DcR2 expression) in two kinds of p53-deficient cell lines.As we expected, overexpression of RKIP could suppress the cell proliferation, whereas RKIP knockdown could promote the cell proliferation. |
| Regulatory pathway: | RAS-RAF-ERK |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | KRAS-Raf-MAPK1 |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Western blot |
| Pathway description: | Suppression of Ras-Raf-ERK pathway using Raf kinase inhibitor could induce the cellular senescence .Elimination of RKIP could increase ERK activation and suppress the cellular senescence. Conversely, RKIP overexpression inhibited ERK activation. |
Annotation:
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