| Gene name: | TXNIP |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry//Cell proliferation assay |
| Description: | The results showed that cells expressing VDUP1 arrested cell cycle progression at the G0/G1 phase,displayed the morphology of flat cells, stained positive for SA-β-gal, and formed SAHF 9 days after retroviral infection (5 days post-selection); moreover, molecular markers of senescence, such as increased p53, p21, p16, and decreased p-Rb levels, were also detected after VDUP1 overexpression. |
| Target gene: | FOXO3A//MIR-17-5P |
| Official symbol(s): | FOXO3A//MIR-17-5P |
| R-AG-Target gene: | Upregulation//Downregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | Luciferase reporter assay//qRT-PCR |
| Target gene description: | Mutation of the FOXO-binding element decreased about 2.2- fold luciferase activity in senescent cells as compared with the wild-type construct. In contrast, the luciferase activity from the mutant construct slightly decreased as compared with the wildtype construct in young cells.To demonstrate that miR-17-5p interacts with specific target sequence localized in this region of VDUP1 3-UTR, an additional reporter construct was generated in which the 7-bp “seed” sequence (CGUGAAA) of two putative miR-17-5p target sites were mutated using PCR.The resulting construct, pVDUP1–3UTR/ miR-17- 5p, was transfected into young and senescent cells; this mutation dramatically increased luciferase activity in young cells, whereas only a slight elevation was observed in senescent cells.Down-regulation of miR-17-5p Expression Is Associated with the Increase in VDUP1 Expression in Senescent Cells |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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