| Gene name: | AKT1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Embryo |
| Cell name: | MEF |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//BrdU assay |
| Description: | WT MEFs began senescing after passage, whereas Akt1/2 DKO MEFs began senescing after passage.This was also confirmed by the cells’enlarged and flattened cell morphology (data not shown),by SA-β-Gal staining,and by BrdU incorporation. |
| Target gene: | ROS//FOXO//SESTRIN 3 |
| Official symbol(s): | ROS1//FOXO//SESTRIN 3 |
| R-AG-Target gene: | Upregulation//Downregulation//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunostaining//qRT-PCR |
| Target gene description: | We found that Akt1/2 DKO MEFs had significantly lower ROS levels compared with WT MEFs, whereas cells expressing activated Akt and Pten-/- cells had significantly higher levels of ROS.FoxO transcriptional activity was elevated in Akt1/2 DKO MEFs.We found that sestrin 3 (Sesn3) expression was highly induced by activated FoxO.Activated Akt induces ROS by increasing oxygen consumption combined with the inhibition of FoxO transcription factors. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...