| Gene name: | CXCL1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | NOF150,NOF151 |
| Gene ID: | 2919 |
| Category: | protein coding |
| Phenotype: | Ovarian cancer |
| Experimental category: | L |
| PMID: | 17060621 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Western blot//Immunostaining |
| Description: | We found that the cells became enlarged andflattened, showing growth arrest and increased SA-β-gal activity after 10 days of treatmentas compared with cells grown in normal medium without Gro-1,cells cultured in serum-free medium, or cells treated with the same concentration of mouse IgG.Gro-1 treatment of NOF151 cells led to expression of HP1β in granular foci and marked increases in cytoplasmic p16INK4A levels. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Cell morphological analysis//SA-β-gal activity assay//Western blot |
| Pathway description: | Treatment of parental NOF150 cells with Gro-1 led to strong blue staining on day 10, demonstrating activation of cellular senescence by Gro-1, but disruption of p53 suppressed or eliminated this effect.Fibroblasts immortalized by the introduction of p53siRNA and hTERT in particular completely lost the senescent response to Gro-1 .Disruption of p53 also markedly attenuated p16INK4Aexpression, particularly in the presence of hTERT. |
Annotation:
Loading,please wait...