| Gene name: | BMI1 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay |
| Description: | We infected near-senescent cells with pBabe-Bmi-1.Replicative life span increased by ~4 population doublings (PD),relative to control cells.Consistent with these results,Bmi-1 transiently lowered the fraction of cells expressing SA-β-Gal,a marker of the senescent phenotype. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Most cells in Bmi-1-overexpressing cultures did not express p16.Western analysis confirmed that Bmi-1 overexpression rapidly decreased p16 levels in presenes -cent WI-38 cultures;moreover, p16 levels increased modestly when these cultures senesced. |
| Regulatory pathway: | PRB |
| R-AG-Pathway: | -- |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | Cell counting |
| Pathway description: | By contrast, Bmi-1 had no significant effect on the replicative life span of cells expressing E7(pRb eliminated),E6 and E7, or SV40 large T antigen. |
Annotation:
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