| Gene name: | WSB1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Embryo |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Consistent with previous publications, expressing H-RasV12 in MEFs (H-RasV12-MEFs) caused cellular senescence, characterized by enhanced senescence-associated β-galactosidase (SA-β-gal) staining, flattened cell morphology, and upregulation of p53, p21, and p16. |
| Target gene: | ATM |
| Official symbol(s): | ATM |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Ubiquitylation |
| Target gene experiment: | SA-β-gal activity assay//Colony formation assay |
| Target gene description: | Interestingly, we observed decreased ATM staining and levels in cells overexpressing WSB1.These results led us to hypothesize that WSB1 might negatively regulate A TM levels and that ATM might be a major target of WSB1 in overcoming OIS.We also obtained similar results using ATM+/+ and ATM?/? immortal MEFs. These results suggest that A TM is a major target of WSB1 in inducing cell transformation. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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