| Gene name: | MAD2L1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Consistent with this observation we scored in IMR90 human fibroblasts with MAD2 depletion roughly 70% of metaphase spreads with prematurely separated sister chromatids.At this time the majority of the human fibroblasts showed enlarged and flattened morphology a typical feature of cellular senescence.Moreover, these IMR90-siMAD2 cells showed nuclei alterations such as binucleate or multinucleated nuclei in 48% of the cells. |
| Target gene: | P21//P14 |
| Official symbol(s): | P21//P14 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | Real time RT-PCR revealed a high increase (seven fold) of p21waf1 mRNA in primary human fibroblasts with MAD2 haploinsufficiency when compared to IMR90-siGFP control cells. Also MCF10A-siMAD2 cells showed p21waf1 increased levels but to a lesser extent than those found in IMR90-siMAD2 cells.As expected western blotting confirmed that p21waf1 protein was significantly higher in siMAD2 than in IMR90-siGFP transfected cells. As expected western blotting confirmed that p21waf1 protein was significantly higher in siMAD2 than in IMR90-siGFP transfected cells.At 72 hours post-transfection, p14ARF mRNA was highly increased in IMR90 human fibroblasts .Similarly, western-blotting experiments showed a high increase of p14ARF protein levels in IMR90-siMAD2 cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | RT-PCR |
| Pathway description: | When p53 was post-transcriptionally silenced in IMR90-siMAD2 cells, expression level of the p21waf1 gene resulted similar to that present in IMR90-siGFP control cells, as assessed by Real time RT-PCR analysis. |
Annotation:
Loading,please wait...