| Gene name: | LIN9 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Embryo |
| Cell name: | BJ,MEF |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | After 10–13 days in culture, LIN9-depleted cells exhibited a large and flat morphology, a phenotype that is commonly observed in senescent cells.Indeed, staining for senescence-associated β-galactosidase activity, a marker of senescent cells, confirmed senescence after loss of LIN9.Two weeks after deletion of LIN9, MEFs became senescent, as previously described . |
| Target gene: | P16//P21 |
| Official symbol(s): | P16//P21 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Immunoblot analysis showed induction of the cell cycle inhibitors p16INK4a and p21Waf1 in LIN9-depleted cells.Similar to what was observed in human cells, loss of LIN9 in two different MEF clones was also accompanied by induction of p16INK4a and p21Waf1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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