| Gene name: | TERT |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Kidney |
| Cell name: | RPTEC |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Immunostaining |
| Description: | RPTEC/TERT1 cells were propagated as a mass culture because of poor cloning efficiency. They showed growth rates of 0.25 PD/day and a population doubling time of ～96 h, comparable to the normal counterpart, and have now accomplished >90 PDs, corresponding to more than threefold life span extension since normal RPTECs enter growth arrest after 24 ± 3 PDLs.RPTEC/TERT1 cells retained morphology very similar to early-passage cells as well as few SA-β-Gal-positive cells. Interestingly, a considerable fraction of ～20% positive cells was observed also in early-passage cells.γ-H2AX focus formation in RPTEC/TERT1 cells was not reduced to early-passage cell levels, with a number of 7.1 foci per cell. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...