| Gene name: | BRCA1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Mammary Gland |
| Cell name: | HMEC |
| Gene ID: | 672 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 26106036 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | This premature growth arrest (M*) was observed across multiple patient-derived BRCA1mut/﹢HMECs with different BRCA1 mutations and was observed in BRCA1mut/﹢HMECs well . Cells in M* displayed the senescent phenotype, characterized by enlarged, flattened morphology and positive staining for SA-β-galactosidase. |
| Target gene: | CYCLIN A //SIRT1 |
| Official symbol(s): | CYCLIN A //SIRT1 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Levels of cyclin A were significantly decreased in senescent BRCA1mut/﹢HMECs compared with WT HMECs.Examination of SIRT1 levels in HMECs from BRCA1-mutation carriers revealed significantly reduced protein expression insenescent cells. |
| Regulatory pathway: | PRB |
| R-AG-Pathway: | -- |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | Knockdown |
| Pathway description: | Compared with control, knockdown of pRb in BRCA1mut/﹢HMECs led to an increase in replicative potential, indicating that pRb activity was mediating premature senescence. |
Annotation:
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