| Gene name: | TP53 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SH-EP |
| Gene ID: | 7157 |
| Category: | protein coding |
| Phenotype: | Neuroblastoma |
| Experimental category: | HL |
| PMID: | 17974978 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Knockdown |
| Description: | This arrest appeared to be permanent for p53 wild-type cells, the majority of which stayed on the plates during the whole duration of the experiment. Arrested p53 wild-type cells expressed acidic β-galactosidase, a marker of cellular senescence. In contrast, however, in p53-deficient cells after DNA damage, growth arrest was either temporary (as judged by reappearance of S-phase) or incomplete. Resumption of cell cycle progression was accompanied by gradual accumulation of cells with both sub-G1 and polyploid (>4C) DNA content. Simultaneously, numerous multinucleated cells and cells with multiple micronuclei appeared (data not shown) and the population underwent significant cell loss: By the end of the period of observation, most parts of the plate surface contained no cells. At the same time, in treated populations of p53-deficient cells, we observed formation of multiple colonies of rapidly dividing cells with no traces of senescence-specific β-galactosidase. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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