| Gene name: | RASSF1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Tumor tissue |
| Cell name: | A549 |
| Gene ID: | 11186 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 19223555 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | Conditional RASSF1A expression arrested A549 cells in the G1 phase of the cell cycle.RASSF1A-mediated cell cycle arrest in A549 human lung cancer cells was accompanied by morphologic and biochemical features of sensescence.Moreover, tumors with conditionally expressed RASSF1A exhibited SA-β-gal activity, which serves as an indicator for senescence occurring in vivo, as well as enhanced p21Cip1/Waf1 expression. |
| Target gene: | P21/WAF1 |
| Official symbol(s): | P21/WAF1 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | In agreement with our findings in A549 cells, RASSF1A expression increased endogenous p21Cip1/Waf1 levels in p21Cip1/Waf1-proficient HCT116 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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