| Gene name: | TP53 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Saos-2 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Immunostaining |
| Description: | Bright‐field microscopic images of COE alone, COE + vector and COE + p53 (V138) SKOV‐3 cells at 32 °C and 37 °C showed growth arrest in COE + p53 (V138) at 32 °C only .We examined HP1γ nuclear heterochromatin foci in p53 (V138)‐transfected COE Saos‐2 and SKOV‐3 cells (data not shown) at 32 °C and 37 °C. HP1γ foci were observed only at 32 °C in both the cell lines. We observed β‐galactosidase positive senescence in p53 (V138)‐transfected COE cells at 32 °C. Whereas a significant increase in β‐galactosidase positive cells was seen in p53 (V138)‐transfected cells cultured at 32 °C, the number of Ki‐67 positive cells was low. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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