| Gene name: | TIGAR |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | OVCA420 |
| Gene ID: | 57103 |
| Category: | protein coding |
| Phenotype: | Ovarian cancer |
| Experimental category: | HL |
| PMID: | 31508509 |
| Experiment: | SA-β-gal activity assay//EdU assay//Knockdown//Flow cytometry |
| Description: | Within 3?h of the pulse-chase, most of the cells in TIGAR-proficient, scrambled siRNA-transfected cells were progressed into G2/M phase whereas the majority of TIGAR KD cells remained in S-phase. In addition to delay in S-phase, G2/M transition is also affected in TIGAR KD cells because remarkably lower number of phospho-H3-positive M-phase cells were observed in TIGAR KD cells compared to TIGAR-proficient counterparts. we observed an increase in SA-β-gal staining in the TIGAR KD cells and the extent of cells undergoing senescence is inversely correlated with TIGAR expression level. Similar results were observed in A2780 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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