| Gene name: | RHEB |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Western blot//Cell morphological analysis |
| Description: | Rheb expression causes morpho logical changes (data not shown), induction of senescence-associated β-galactosidase (SA β-Gal) ,early growth arrest (Vector vs. Rheb, P < 0.0001),and p16 protein induction . |
| Target gene: | P53//MTORC1 |
| Official symbol(s): | P53//MTORC1 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//Flow cytometry//Western blot |
| Target gene description: | As expected, these effects depended on p53 and did not occur in the context of c-Myc expression.Notably, pharmacologic inhibition of mTORC1 with rapamycin could prevent many phenotypic changes associated with senescence induced by Akt and Rheb and had a partial effect on Ras-induced senescence. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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