| Gene name: | GPC3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HuH-7,HepG2 |
| Gene ID: | 2719 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 21847365 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Suppression of GPC3 caused a significant increase in G1 peak in HepG2 cells (P =0.04 compared with siRNA-N), together with a decrease in S-phase cells.Transfection with siRNA-GPC3 alone caused marked accumulations of SA-β-Gal in both cell lines. |
| Target gene: | TGF-β2 |
| Official symbol(s): | TGFB2 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Semi-RT-PCR |
| Target gene description: | A member of theTGFBR pathway, TGF-β2 (but not TGF-β1 or TGF-β3), was significantly upregulated by GPC3 suppression in both Huh7 and HepG2 cells.The up-regulation of TGF-β2 by siRNA-GPC3 was independently validated using semiquantitative RT-PCR and Western blot analysis to confirm the negative correlation at both transcript and protein levels, respectively. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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