| Gene name: | RBM38 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SMMC-7721,HepG2 |
| Gene ID: | 55544 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | L |
| PMID: | 30176896 |
| Experiment: | SA-β-gal activity assay//CCK-8 assay//Colony formation assay |
| Description: | The proportion of cells that were positive for β-galactosidase activity, an indicator of cell senescence, was significantly increased in the HepG2-RBM38-OE (p = 0.0077) and SMMC7721 RBM38-OE cell lines (p = 0.0421) compared to the corresponding controls.Up-regulation of RBM38 led to significantly decreased cell proliferation in HepG2-RBM38-OE cells (p = 0.032) and SMMC7721-RBM38-OE cells (p = 0.044) compared to their corresponding control cells. The colony formation assay showed that when RBM38 was over-expressed, the colony number and size were significantly reduced in HepG2-RBM38-OE (colony number, p = 0.01145; colony size, p = 0.0001) and SMMC7721-RBM38-OE cell lines (colony number, p = 0.0116; colony size, p = 0.0001) when compared to their corresponding control cells . |
| Target gene: | P53//MDM2 |
| Official symbol(s): | P53//MDM2 |
| R-AG-Target gene: | Upregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Luciferase reporter assay |
| Target gene description: | Up-regulation of RBM38 lead to a significant increase in wtp53 and inhibition of mdm2 protein levels compared to their corresponding controls.We found that the luciferase activity for a reporter carrying mdm2 3′-UTR-B and -C was significantly repressed by RBM38 in SMMC7721-RBM38-OE cells. By contrast, the mdm2 3′-UTR-A was not responsive to RBM38. In Hep-G2-RBM38-OE cells, the luciferase activities for reporters carrying mdm2 3′-UTR-A, -B and -C were significantly repressed by RBM38. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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