| Gene name: | TERT |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//Flow cytometry//TUNEL assay//CCK-8 assay |
| Description: | Compared with the control group, the downregulation of hTERT induced cellular senescence, decreased cell proliferation and the percentage of cells in the S phase and increased the percentage of apoptotic MSCs. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | We found that the downregulation of hTERT significantly decreased p-PI3K and p-AKT expression in the MSCs, whereas the upregulation of hTERT significantly increased p-PI3K and p-AKT expression in the MSCs; no changes were observed in the levels of total PI3K and AKT in either group.The MSCs were treated with LY294002 (Sigma-Aldrich), a PI3K inhibitor, and the hTERT expression levels were then measured by western blot analysis. The results revealed that LY294002 significantly inhibited hTERT expression. |
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