| Gene name: | PTEN |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BM-MSC |
| Gene ID: | 5728 |
| Category: | protein coding |
| Phenotype: | Systemic Lupus Erythematosus |
| Experimental category: | L |
| PMID: | 25649549 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Knockdown |
| Description: | There were less SA-β-gal-positive cells when PTEN expression was knocked down in BM-MSCs from SLE patients, but it has less effect in the control group’s BM-MSCs. The further quantitative analysis indicated the number of SLE patients’ BM-MSCs increased in si-PTEN-transfected group compared to the untreated group from the third day .Cell-cycle analysis revealed that G1 phase arrest was observably reversed in si-PTEN-transfected SLE BM-MSCs. |
| Regulatory pathway: | P27 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | CDKN1B |
| Pathway experiment: | Western blot//Immunofluorescence |
| Pathway description: | In the BM-MSCs from SLE patients, we found that the expression of PTEN was up-regulated, and the phosphorylation of Akt was reduced. Meanwhile, the higher expression of PTEN and the lower expression of p-Akt in SLE BM-MSCs were confirmed by immunofluorescence. The expression of cell-cycle regulator p27kip1 was determined. The results showed that p27kip1 increased markedly in BM-MSCs from SLE patients and nuclear fluorescence intensity was enhanced. The expression of p27kip1 decreased significantly in SLE BM-MSCs transfected with si-PTEN . |
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