| Gene name: | KLF6 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LN-229,BTSC233 |
| Gene ID: | 1316 |
| Category: | protein coding |
| Phenotype: | Glioblastoma |
| Experimental category: | HL |
| PMID: | 28166199 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Prolonged expression of KLF6-wt, but not KLF6-sv1, induced a senescent-like phenotype in both LN229 and BTSC23 cells, highlighted by β-galactosidase staining. Cell cycle analysis revealed accumulation of cells in phase G1–G0 and concomitant reduction of cells in phases S and G2/M, upon KLF6-wt expression.In contrast, KLF6-sv1 overexpression prolonged S-phase. In BTSC23 cells, KLF6-wt overexpression led to accumulation of cells in G2/M .Consistent with the G1 arrest observed in LN229 cells, KLF6-wt overexpression led to upregulation of CDKN1A expression in LN229 and BTSC23 cells . |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Downregulation of NF-κB targets MMP9, OLIG2 and YKL40 was confirmed by western blot. |
Annotation:
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