| Gene name: | NCOA4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Western blot//Knockdown//Immunostaining |
| Description: | NCOA4?/? cells showed increased activation of the apical DDR kinases ATM and ATR, accumulation of nuclear foci stained for pS/TQ ATM/ATR substrates, and increased phosphorylation of p53 on S15. Furthermore, NCOA4?/? cells displayed an increased number of γ-H2AX- and 53BP1-positive cells compared to NCOA4+/+ MEFs.Consistently, NCOA4?/? MEFs displayed reduced PDL (population doubling level) accumulation compared to both NCOA4+/+ and NCOA4+/? cells, rapidly exhausted replicative potential, and after four passages, entered premature senescence, as shown by increased β-galactosidase staining (p < 0.001). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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