| Gene name: | COPS5 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Western blot |
| Description: | Under the microscope, 4OHT‐treated cells appeared flatter, a typical feature of senescent cells. We, therefore, assayed for senescence‐associated (SA) β‐galactosidase (Gal) activity, another marker of premature senescence.Lower panels show that 4OHT‐treated CSN5f/‐p53?/?Ras+CRE‐ER MEFs were positive for SA‐β‐Gal activity.As cells underwent senescence, the expression of the CDK inhibitors p21, p27, and p16 was upregulated and hypo‐phosphorylated Rb protein was accumulated, whereas the level of Skp2 was maintained. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | -- |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | Although the total expression levels of ERK1, ERK2, and Akt were maintained, their activating phosphorylation was modulated by knockout of the CSN5 gene. The Activate form of ERK1 and 2 was reduced, whereas the activating phosphorylation of Akt (serine 473 and threonine 308) was increased after treatment with 4OHT. Furthermore, the phosphorylation of certain substrates of Akt was enhanced in cells deprived of CSN5.We observed the same phenotype when we used a chemical inhibitor more specific to PI3 kinase, LY294002. |
Annotation:
Loading,please wait...