| Gene name: | FASN |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HSC |
| Experiment: | RT-PCR//SA-β-gal activity assay//Western blot//Knockdown |
| Description: | FASN knockdown in senescent HSCs prevented the senescent-induced cell cycle arrest, in addition to preventing the upregulation of different markers of senescence such as SA-β-gal activity, p53, p16 and p21. Notably, FASN knockdown also inhibit the increase in the expression of mRNA levels of SASP factors IL1A, IL1B and IL6 in HSCs. |
| Regulatory pathway: | MTOR |
| R-AG-Pathway: | -- |
| Official symbol(s): | MTOR |
| Pathway experiment: | Western blot |
| Pathway description: | To our surprise,mTOR activity was repressed during senescence as shown by a decrease in the phosphorylation levels of some of its downstream targets: S6, 4EBP1 and AKT. Remarkably, senescent cells treated with C75 were able to restore mTOR signalling at levels similar to control. |
Annotation:
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