| Gene name: | NPM1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//BrdU assay |
| Description: | Fibroblasts that overexpressed NPM remained viable for several days but did not incorporate bromodeoxyuridine.Morphologically the cells became flat, enlarged and positive for acidic β-galactosidase (SA-β-Gal), a marker of senescence. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Cell counting//Western blot//qRT-PCR |
| Target gene description: | The physical association and colocalization of NPM and p53, and its ability to induce cellular senescence in a p53-dependent manner, suggest that NPM might regulate p53 activity directly.GFP–NPM inhibited the growth of human WI38 cells and caused a marked upregulation in the amounts of p53, hdm2 and p21 proteins.Increased transcription of hdm2 and p21 was con-firmed at the mRNA level by quantitative polymerase chain reaction. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...