| Gene name: | GNMT |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HuH-7-GNMT,HuH-7-GFP,HepG2 |
| Gene ID: | 27232 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | L |
| PMID: | 22160218 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//MTT assay |
| Description: | The growth rate of HuH-7-GNMT cells was significantly slower than that of HuH-7-GFP cells. Similar results were also observed in HepG2 cells overexpressing GNMT. Cell cycle analysis showed that, 36 h after the cells entered the cell cycle, the percentages of cells in the G2/M phase for HuH-7-GNMT cells and HuH-7-GFP cells were 23.8% and 10.4%, respectively.Furthermore, SA-β-gal assay demonstrated that HuH-7-GNMT cells had asignificantly higher rate of positive staining than HuH-7-GFP cells. |
| Target gene: | DEPTOR |
| Official symbol(s): | DEPTOR |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | FRET-AB assay//Co-IP |
| Target gene description: | Immunoprecipitation of either HAtagged DEPTOR or endogenous DEPTOR coprecipitated FLAG-tagged GNMT. In addition, we detected endogenous DEPTOR in GNMT immunoprecipitants prepared from mouse liver. The results showed that the FRET efficiency between full-length GNMT and DEP domains of DEPTOR decreased significantly. In addition, a >50% decrease of the FRET efficiency was found between full-length DEPTOR and the N-terminal of GNMT. |
| Regulatory pathway: | MTOR-RAPTOR |
| R-AG-Pathway: | Activation |
| Official symbol(s): | MTOR-RPTOR |
| Pathway experiment: | Western blot |
| Pathway description: | The results showed that overexpression of GNMT led to increases of both 4E-BP phosphorylation and cell size. In addition, overexpression of DEPTOR in HuH-7-GNMT stable cells resulted in the neutralization of the effect of GNMT on 4E-BP phosphorylation. |
Annotation:
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