| Gene name: | SEMA4C |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MDA-MB-231 |
| Gene ID: | 54910 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 31308149 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown//Cell counting//Immunostaining |
| Description: | Cell counting assays demonstrated that knockdown of SEMA4C significantly inhibited 24 MDA-MB-231 cellular growth ability. Cell immunohistochemistry also indicated that MDA-MB-231 cells expressing shSEMA4C showed a >50% reduction in proliferation rate as assessed by anti-human PCNA staining.However, in the following days, some gradually acquired a flattened and enlarged cell shape, thus implying a pro-senescent non-apoptotic state.The senescent marker of SA-β-gal increased significantly in MDA-MB-231cells transfected with either siSEMA4C or siPLEXINB2 compared with control cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | The ratio of phospho-p21Cip1–Thr145 to total p21Cip1 increased in the MDA-MB-231 cells transfected with either siSEMA4C or siPLEXINB2,suggesting that knockdown of SEMA4C or PLEXINB2 induced activation of p53, followed by activation of p21Cip1. |
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