| Gene name: | CDKN2A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: | The 2BS/p16 cells showed increasing gross en- largement, flattening, and accumulation of granular cytoplas- mic inclusions.Only sporadic SA-β-gal-positive cells were seen in 2BS/ASp16 (A5, PD45) and young (PD29) cells, whereas almost all of 2BS/p16 (P3, PD43) cells were strongly stained, as were the senescent 2BS control cells (PD58).p16INK4acan inhibit the activity of CDKs, thereby blocking the entry of proliferating cells from G1to S phase. |
| Regulatory pathway: | P16-CDK4-PRB |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN2A-CDK4-RB1 |
| Pathway experiment: | Western blot |
| Pathway description: | p16INK4acan inhibit the activity of CDKs,thereby blocking the entry of the proliferating cells to S phase through the p16INK4a/CDK4/cyclin D/pRB pathway. The function of p16INK4a is to maintain pRB in its hypophosphorylated Activate state.pRB phosphorylation status in 2BS/ASp16 (A10, PD50) cells (lane 1) is similar to that in young cells (PD25) (lane 2), which is considerably higher than that of senescent cells . |
Annotation:
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