| Gene name: | TAX |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HeLa |
| Experiment: | Western blot |
| Description: | We use the degradation of I-kBa as an indicator of NF-kB activation and the decrease in cyclin B1 and Skp2 levels with concomitant increase in the levels of p21 and p27 as surrogate markers for senescence induction.Wild-type Tax (WT) and those Tax mutants (V89A and M47) that are potent activators of NF-kB greatly reduced the levels of cyclin B and Skp2, and dramatically elevated p21 and p27 expression. |
| Target gene: | RELA//HBZ |
| Official symbol(s): | RELA//HBZ |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown//Luciferase reporter assay |
| Target gene description: | Most interestingly, knockdown of RelA and to a rather limited extent, that of RelB, but not that of c-Rel or p100 prevented Tax-induced senescence.This is evidenced by the proliferation of EGFP+ HeLa-G/RelAKD cells and the absence of p21 and p27 up-regulation therein after Ad-Tax transduction.Like native HBZ, Flag-HBZ efficiently inhibits NF-kB activation by Tax. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Tax mutants that are impaired in NF-kB activation , in comparison, had only moderate or no effect on cyclin B, Skp2, p21, or p27. |
Annotation:
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