| Gene name: | NPM1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MIP101,RKO,HCT116 |
| Gene ID: | 4869 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 23536448 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Based on SA-β-gal staining, there were more senescent cells among all CRC cell lines transfected with NPM1 siRNA compared to those transfected with scramble siRNA. By MUG assay, a 20%, 35% and 45% increase in cellular senescence were observed in MIP101, RKO and HCT116 cells following NPM1 siRNA knockdown, respectively. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Immunostaining//Western blot |
| Target gene description: | Interestingly, remarkable increases in the expression of p53 were noted after NPM1 gene silencing in all three CRC cell lines by immunofluorescence staining and confirmed in MIP101 cells by immunoblotting. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...