| Gene name: | LMNA |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Fibroblast |
| Gene ID: | 4000 |
| Category: | protein coding |
| Phenotype: | Lipodystrophic syndromes |
| Experimental category: | L |
| PMID: | 17612587 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | In contrast, SA-β-galactosidase activity, assessed at pH 6, was absent in control cells up to passage 14, but present, even at early passages, in fibroblasts with LMNA mutations or treated with PIs. |
| Target gene: | P16//P21 |
| Official symbol(s): | P16//P21 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Immunostaining |
| Target gene description: | P16INK4aand p21WAF1,two cell cycle checkpoint inhibitors that participate in the setup of the senescence program,were overexpressed by 250–400% in fibroblasts bearing LMNA mutations at passages 9–15.Dichloro- fluorescein oxidation was increased two- to fivefold in fibroblasts with LMNA mutations at passages 6 8, as compared to control cells.Microscopic examination confirmed the increased production of ROS in fibroblasts with LMNA muta-tions,as compared to control fibroblasts. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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