| Gene name: | TNF |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ISO-HAS |
| Experiment: | SA-β-gal activity assay//RT-PCR |
| Description: | Treatment with 10 ng/mL TNF-α for 48 h increased the proportion of SA-β-gal-positive cells in the endothelial cell culture.Gene expression of SHC1 and GLB1 increased at TNF-α concentrations above 1 ng/mL,and the significance of this effect was reached for all three genes at 10 ng/mL. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We used Western blotting to examine the protein expression levels of p21 in endothelial cells following 10 ng/mL TNF-α treatment for 8 h. TNF-α exposure induced the expression of p21 in ISO-HAS cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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