| Gene name: | POT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MRC-5,WI-38,NHF |
| Experiment: | SA-β-gal activity assay//Knockdown//BrdU assay |
| Description: | The shRNA knockdown of endogenous POT1v1 or POT1v5 induced cellular senescence, characterized by cell growth arrest and SA-β-Gal activity, in normal human fibroblast strains MRC-5, NHF, and WI-38.The significant decrease in bromodeoxyuridine incorporation was associated with sh-v1 induced shsenescence(1.9% ,compared with 43.9% in control cells）. |
| Regulatory pathway: | P53//P16 |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | TP53//CDKN2A |
| Pathway experiment: | Western blot |
| Pathway description: | The p53 dependence of sh-v5–induced senescence in this experiment was consistent with the Western blot results of cellular senescenceregulatory factors in normal human fibroblasts. Both sh-v1 and sh-v5 led to the increase in Ser15-phosphorylated p53 and the up-regulation of p21WAF1, an effector of p53-mediated cellular senescence.In contrast, only sh-v1 induced the expression of p16INK4A, another major effector for cellular senescence in human cells. |
Annotation:
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