| Gene name: | TAT |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | In control cells, the percentage of senescent cells X-gal stained was 8.6±2.2% and 9.3 ± 2.2%, after 10 and 20 days of culture, respectively. After 10 days, senescence was increased in cells treated with Tat+Nef, and after 20 days, its level was of 17.1 ± 1.6%, 18.0 ± 4.1% and 20.2 ± 3.7% in Tat-, Nef- and Tat+Nef-treated cells, respectively. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Treatment with Tat but not Nef resulted in an increased nuclear translocation of the activated pro-inflammatory and pro-senescent transcription factor NF-κB, as shown by the accumulation of the activated phospho-Ser 536 form of p-65 in the nucleus . |
Annotation:
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