| Gene name: | CAV1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A549 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown//Cell counting//Colony formation assay |
| Description: | Unexpectedly, Cav-1 knockdown increased β-gal positivity and changed the cellular morphology from a normal epithelial shape to a fried egg-like shape.Cav-1 knockdown significantly decreased cellular proliferation and colony-forming capacity compared with the si-control-treated cells. |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | Therefore, we measured SIRT1 activity by measuring acetylated p53 expression after Cav-1 knockdown in A549 cells using immunoblotting. The acetylation of p53 was gradually increased with time after Cav-1 knockdown. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | SA-β-gal activity assay//Knockdown//Western blot//Cell counting |
| Pathway description: | Analyses of cell numbers, β-gal staining positivity, and p-pRb, p53, and p21 expression levels showed that p53 or p21 knockdown prevented Cav-1 knockdown-induced cellular senescence. |
Annotation:
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