| Gene name: | CEBPG |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//qRT-PCR//Knockdown |
| Description: | MEF cultures contained many cells with a flattened morphology and vacuolated cytoplasm, indicative of premature entry into senescence. This observation was confirmed by SA–β-Gal staining assays, which showed that mutant MEFs contain ～4-fold more senescent cells than WT MEFs.We used qPCR to evaluate expression of candidate SASP genes (GROα/Cxcl-1, Cxcl2, Ccr-1, Il6, Il1a, and Il1b)in Cebpg-/-MEFs and RasV12-expressing Cebpb-/-MEFs. Each gene was induced in C/EBPγ-deficient MEFs compared to WT cells. |
| Target gene: | C/EBPβ |
| Official symbol(s): | CEBPB |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//SA-β-gal activity assay//qRT-PCR |
| Target gene description: | C/EBPβ knockdown with two independent shRNAs increased the proliferative capacity of Cebpg-/-MEFs, as judged by cell densities 7 days after plating. C/EBPβ ablation also significantly reduced the proportion of senescent cells and reversed the aberrant expression of SASP genes Cxcl1 and Cxcl2. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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