| Gene name: | SIRT6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Primary human keratinocyte |
| Experiment: | Knockdown//SA-β-gal activity assay//Western blot |
| Description: | SIRT6 knockdown significantly increased senescence of primary keratinocytes,and this effect was reversed by depletion of RELA by RNAi. |
| Target gene: | H3K9 |
| Official symbol(s): | EHMT1 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | CHIP//Western blot |
| Target gene description: | ChIP analysis revealed that H3K9 acetylation is induced following TNF-a treatment in SIRT6-proficient control cells at the promoters of multiple NF-kB target genes, consistent with transcriptional induction. In cells depleted of SIRT6, H3K9 was hyperacetylated at these promoters in response to TNF-a. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Luciferase reporter assay//Knockdown |
| Pathway description: | SIRT6 depletion led to constitutive NF-kB reporter gene activity, which, upon TNF-a treatment,was further enhanced to levels considerably higher than in SIRT6-proficient control cells . |
Annotation:
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