| Gene name: | NSUN2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Knockdown of NSun2 inhibited cell growth and increased the proportions of cells displaying the protein marker senescence-associated (SA)-β-galactosidase (~37.5% vs. ~91.0%), while overexpression of NSun2 promoted cell growth and reduced SA-β-galactosidase activity (~33.5% vs. ~10.6%). |
| Target gene: | P27//CDK1//CDC25C |
| Official symbol(s): | P27//CDK1//CDC25C |
| R-AG-Target gene: | Downregulation//Upregulation//Upregulation |
| Subcategory: | Methylation |
| Target gene experiment: | Western blot |
| Target gene description: | Infection with the NSun2-expressing lentiviruses reduced the levels of p27 by ~70%, while infection with NSun2 shRNA-expressing lentiviruses increased the levels of p27 by ~5.1 fold.In contrast, the levels of proteins CDK1 and CDC25C increased in cells with overexpressed NSun2 (by ~2.8 fold for CDK1; by ~1.5 fold for CDC25C) but decreased in cells with silenced NSun2 (by ~70% for both CDK1 and CDC25C). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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