| Gene name: | LMNA |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Umbilical cord blood |
| Cell name: | MSC |
| Experiment: | Western blot//SA-β-gal activity assay//MTT assay |
| Description: | Immunoblots confirmed the over-expression of GFP-progerin and increased expression of p16INK4a, a marker of senescence in hMSCs;Induction of cellular senescence was also confirmed by the increase in SA-β-gal activity;MTT activity was decreased in response to progerin over-expression, indicating decreased proliferative activity. |
| Target gene: | MCP-1//NF-?B |
| Official symbol(s): | CCL2//NFKB1 |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//ELISA//Western blot//Immunostaining |
| Target gene description: | Except for MCP-1, most cytokines that have been reported to increase in senescent fibroblasts were not increased in senescent hMSCs expressing progerin.The secretion of MCP-1 was also increased in the CM from progerin-expressing cells.The activation of NF-?B was also increased in response to progerin expression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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