| Gene name: | HIC1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PANC-1,BxPC-3,AsPC-1 |
| Gene ID: | 3090 |
| Category: | protein coding |
| Phenotype: | Pancreatic cancer |
| Experimental category: | L |
| PMID: | 22552606 |
| Experiment: | MTT assay//SA-β-gal activity assay//Flow cytometry |
| Description: | Restoration of HIC1 function with 5-aza-dC treatment leaded to a reduction in cell proliferation, obvious cell senescence, cell cycle arrest and apoptosis, accompanied with acetylated p53 and p21WAF1 of Cip1 upregulation.Compared to untreated cells, cell proliferation of PANC-1, BXPC-3 and AsPC-1 treated with 5-aza-dC were significantly repressed . After treated with 5-aza-dC, the senescence positive rate of PANC-1, BXPC-3 and AsPC-1 cells were 43.6 ± 4.0, 69.9 ± 3.3 and 12.2 ± 2.2 %, all of which were significantly higher than in corresponding control group.Compared with control group, 5-aza-dC treated cells in G1/G0 phase were notably increased . |
| Target gene: | P53//P21/WAF1//SIRT1 |
| Official symbol(s): | P53//P21/WAF1//SIRT1 |
| R-AG-Target gene: | Upregulation//Upregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//MTT assay//SA-β-gal activity assay//Flow cytometry |
| Target gene description: | After transfected with pCDNA3FlagHIC1, the acetylated p53 and p21WAF1/Cip1expression of PANC-1 cells significantly increased.After transfection with pCDNA3FlagHIC1, the cells demonstrated high-level expression of HIC1 protein and robustly downregulation of endogenous SIRT1 protein, which resulted in marked cell growth inhibition , cell senescence, cell cycle arrest and apoptosis. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...