| Gene name: | THBS1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HPAEC,HAEC |
| Experiment: | PI staining//Flow cytometry//MTT assay//BrdU assay//SA-β-gal activity assay//Cell morphological analysis |
| Description: | We found that TSP1 induced cell cycle arrest of HPAECs, characterized by increased numbers of cells in G1-G0 and decreased numbers of cells in S and G2-M; TSP1 significantly inhibited HPAEC proliferation, as detected by trypan blue exclusion,MTT assay , and BrdU incorporation; TSP1 exposure significantly increased the number of SA-β-Gal–positive HPAECs and human aortic endothe-lial cells, which showed concentration dependency,and elicited an enlarged and flattened cell shape characteristic of senescence . |
| Target gene: | NOX1//P53//P21 |
| Official symbol(s): | NOX1//P53//P21 |
| R-AG-Target gene: | Upregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Flow cytometry//qRT-PCR//Western blot//Immunostaining |
| Target gene description: | FACS and mRNA analysis confirmed that TSP1 exposure significantly increased Nox1 abundance.We observed that Nox1 protein abundance was significantly lower in middle-aged TSP1?/?lungs compared to controls;We observed an increase in total p53 and p21cipin middle-aged mice.Moreover, we discovered that TSP1 exposure increased p53 abundance in HPAECs in a time-dependent fashion and increased p53 activation, as evidenced by its increased nuclear localization. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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